Journal: Biomaterials Research

Article Title: Mesenchymal stem cell-derived secretomes-enriched alginate/ extracellular matrix hydrogel patch accelerates skin wound healing

doi: 10.1186/s40824-023-00446-y

Figure Lengend Snippet: Histological analysis of the regenerated wound tissues on day 7 and 14. ( A ) High magnification images of the regenerated dermis stained by H&E and DAPI staining (inset) on day 7. Scale bar is 50 μm. ( B ) Medium magnification images of H&E-stained tissue samples. The yellow triangles suggest the neovessels in the dermis region. Scale bar is 100 μm. ( C ) Low magnification images of H&E samples on day 14. Scale bar is 200 μm. ( D ) High and lower magnification in the regenerated dermis as assessed by Herovici staining. Blue and purple color indicated young and mature collagen fibers, respectively. Scale bar is 100 μm. ( E ) Quantification of cellular recruitment in the dermis area on day 7. ( F ) Quantitative evaluation of the average neovessel size on day 7. ( G ) Measurement of the epidermis thickness and comparison with normal skin epidermis on day 14. ( H ) Quantitative analysis of mature collagen deposition in the regenerated dermis on day 14. Statistically significant difference (*** p < 0.001, **** p < 0.0001)

Article Snippet: Collagen deposition and the degree of maturity were also examined via Herovici (KTHERPT; StatLab, Mckinney, TX, USA) staining.

Techniques: Staining, Comparison

Journal: Journal of Cardiovascular Development and Disease

Article Title: New Insights on the Formation of the Mitral Valve Chordae Tendineae in Fetal Life

doi: 10.3390/jcdd11110367

Figure Lengend Snippet: Methods for measuring perforation dimensions in the two modalities. The perimeter was measured as an approximation of perforation size. Representative image of these measurements in a Movat Pentachrome stained section from 219 gestational days ( A ). Representative image of these measurements in 2D still image from micro-CT from 163 gestational days ( B ). Note the scale bar varies between images.

Article Snippet: Sections were stained with the Russell–Movat Pentachrome Stain Kit (Statlab, McKinney, TX, USA) to determine the localization of ECM proteins using the manufacturer’s instructions.

Techniques: Staining, Micro-CT

Journal: Journal of Cardiovascular Development and Disease

Article Title: New Insights on the Formation of the Mitral Valve Chordae Tendineae in Fetal Life

doi: 10.3390/jcdd11110367

Figure Lengend Snippet: Elastin and collagen fibers first appear as short, sparse fragments before beginning to align in parallel with the chordae. ( A ) Movat pentachrome stained section of a perforation (P) with short elastic fragments (red arrows) and longer fragments (green arrows) beginning to align around it. Image taken at 14X magnification using the Pannoramic MIDI II. ( B , C ) Picrosirius red and hematoxylin-stained sections of a perforation (P) with shorter sparse collagen fibrils and collagen beginning to align parallel to the chordae under brightfield ( B ) and polarized light ( C ). Images were taken on a Nikon Eclipse E600 light microscope (Nikon Instruments Inc., Melville, NY, USA) equipped with a polarizer and an AmScope 10MU1400 digital camera (AmScope, Irvine, CA, USA). All photos were taken from sections from the same third trimester valve (219 days into gestation). The scale bar varies per image.

Article Snippet: Sections were stained with the Russell–Movat Pentachrome Stain Kit (Statlab, McKinney, TX, USA) to determine the localization of ECM proteins using the manufacturer’s instructions.

Techniques: Staining, Light Microscopy

Journal: Frontiers in Physiology

Article Title: Effects of a myostatin mutation in Japanese quail ( Coturnix japonica ) on the physicochemical and histochemical characteristics of the pectoralis major muscle

doi: 10.3389/fphys.2023.1172884

Figure Lengend Snippet: Comparison of muscle fiber composition and muscle fiber cross-sections in pectoralis major muscle between the wild type (WT) and myostatin homozygous mutant (HO) quail lines at 2-month of age. (A) . The percentages of type IIA and IIB muscle fibers in superficial or deep regions. (B) . The cross-sectional areas (CSA) of type IIA and IIB muscle fibers in superficial or deep regions. (C) . The representative images of the myofiber types of PM. Muscle fibers were stained using the myosin ATPase staining kit according to the manufacturer’s instructions, and type IIA muscle fibers stained darker than type IIB muscle fibers. Bars in graphs indicate standard errors. Level of significance: NS, no significant; *, p < 0.05; **, p < 0.01. Scale bars: 100 μm. Asterisk indicates type IIA myofibers and arrowheads are type IIB myofibers.

Article Snippet: To measure fiber characteristics, muscle sections were stained using the myosin ATPase staining kit (KTATP, StatLab, McKinney, TX) following the manufacturer’s instructions.

Techniques: Comparison, Mutagenesis, Staining